Description
This webinar will focus on the recently published study that evaluates the negative impacts of long-term storage of formalin-fixed paraffin-embedded (FFPE) tissue on next-generation sequencing (NGS) by describing how the duration of storage affects NGS quality control metrics, variant detection and tumor mutation burden scores. This webinar will also address how treatment of the DNA isolated from stored FFPE with a DNA repair enzyme might reduce storage-related artifacts and improve the accuracy of variant detection.
Read the article in Clinical Chemistry.
Target audience
This activity is designed for Physicians, Lab supervisors, Lab directors (and/or assistant directors), Lab managers (supervisory and/or non-supervisory), Medical technologists, Point-of-Care coordinators, Pathologists, Toxicologists, Fellows, Residents, In-training individuals, and other laboratory professionals overseeing/conducting within this topic.
Learning objectives
At the end of this session, participants will be able to:
- Describe pre-analytical factors that could negatively affect next-generation sequencing.
- Recognize DNA and sequencing quality metrics that reflect effects of long-term FFPE storage.
- Compare characteristics of artifactual variants found in stored FFPE and bona fide tissue-associated variants.
Faculty
Moderator
Linnea Baudhuin, PhD
Professor of Laboratory Medicine and Pathology, Mayo Clinic
Co-Deputy Editor, Clinical Chemistry
Rochester, Minnesota
Speaker
Rena Xian, MD
Associate Professor, Johns Hopkins University School of Medicine
Baltimore, Maryland
Disclosures and statement of independence
The Association for Diagnostics & Laboratory Medicine is dedicated to ensuring balance, independence, objectivity, and scientific rigor in all educational activities. All participating planning committee members and faculty are required to disclose to the program audience any financial relationships related to the subject matter of this program. Disclosure information is reviewed in advance in order to manage and resolve any possible conflicts of interest. The intent of this disclosure is to provide participants with information on which they can make their own judgments.
The following faculty reported financial relationships:
- Rena Xian, MD
- Honorarium and/or Expenses: Invivoscribe, nRichDx
- Grant and/or Research Support: nRichDx
- Committee, Board, and/or Advisory Board Membership: Roche Diagnostics
The following faculty reported no financial relationships:
- Linnea Baudhuin, PhD
Content validity
All recommendations involving clinical medicine are based on evidence accepted within the profession of medicine as adequate justification for their indications and contraindications in the care of patients; AND/OR all scientific research referred to or reported in support or justification of a patient care recommendation conforms to generally accepted standards of experimental design, data collection, and analysis.
Accreditation statement
This activity will be submitted for 1.0 ACCENT® continuing education credits.
Successful completion statement
Verification of Participation certificates are provided to registered participants based on completion of the activity, in its entirety, and the activity evaluation. For questions regarding continuing education, please email [email protected].
