Integrative assessment of total and intact HIV-1 reservoir by a 5-region multiplexed rainbow DNA digital PCR assay

This webinar was recorded live on February 6, 2025.
It is available on demand through February 28, 2026.
Register above for access.

Description

Here, we present the ‘Rainbow proviral HIV-1 DNA dPCR assay’. It is the first-in-its-kind five-color digital PCR assay that performs a quantitative read-out in one multiplex reaction to obtain two crucial reservoir markers based on 5 regions: total reservoir size and the intact reservoir size.

By participating in this webinar, you’ll equip yourself with the knowledge and tools to stay at the forefront of HIV-1 research. Learn how this innovative assay surpasses the traditional methods and explore its unique ability to target multiple genomic regions in one multiplex assay.

Read the article in Clinical Chemistry.

Target audience

This activity is designed for physicians, lab supervisors, lab directors (and/or assistant directors), lab managers (supervisory and/or non-supervisory), medical technologists, point-of-care coordinators, pathologists, toxicologists, fellows, residents, in-training individuals, and other laboratory professionals overseeing/conducting within this topic.

Learning objectives

At the end of this session, participants will be able to:

• Identify the principles of digital PCR for HIV-1 DNA quantification.
• Set up a multiplex digital PCR assay.
• Describe the impact of shearing on DNA quantification.

Faculty

Moderator

Jason Park, MD, PhD
Editor-in-Chief, Clinical Chemistry
Robin M. Jacoby, Ph.D. Professorship in Biomedical Science
Professor, Pathology and the Eugene McDermott Center for Human Growth and Development
University of Texas Southwestern Medical Center
Dallas, TX

Speaker

Mareva Delporte, MS
PhD Candidate, HIV Cure Research Center
Ghent University
Ghent, Belgium

Disclosures and statement of independence

The Association for Diagnostics & Laboratory Medicine (formerly AACC) is dedicated to ensuring balance, independence, objectivity, and scientific rigor in all educational activities. All participating planning committee members and faculty are required to disclose to the program audience any financial relationships related to the subject matter of this program. Disclosure information is reviewed in advance in order to manage and resolve any possible conflicts of interest. The intent of this disclosure is to provide participants with information on which they can make their own judgments.

The following faculty reported financial relationships:

• Jason Park, MD, PhD
  • Ownership Interest: OncoSeer Dx

The following faculty reported no financial relationships:

• Mareva Delporte, MS

Content validity

All recommendations involving clinical medicine are based on evidence accepted within the profession of medicine as adequate justification for their indications and contraindications in the care of patients; AND/OR all scientific research referred to or reported in support or justification of a patient care recommendation conforms to generally accepted standards of experimental design, data collection, and analysis.

Accreditation statement

This activity is approved for 1.0 ACCENT® continuing education credits. Activity ID #4367. This activity was planned in accordance with ACCENT® Standards and Policies.

Successful completion statement

Verification of Participation certificates are provided to registered participants based on completion of the activity, in its entirety, and the activity evaluation. For questions regarding continuing education, please email [email protected].